Study on the effect of blackcurrant extract – based preservative on model membranes and pathogenic bacteria
Authors:
- Beata Wyżga,
- Magdalena Skóra,
- Sławomir Wybraniec,
- Katarzyna Hąc-Wydro
Abstract
Currant Powder abbr. BCE) was investigated to evaluate its antibacterial effect and to gain an insight into its mechanism of action. The influence of this commercially available formulation on model Escherichia coli and Staphylococcus aureus lipid membranes was studied to analyze its interactions with membrane lipids at a molecular level. The mixed lipid monolayers and one component bacteria lipid films were used to investigate the effect of BCE on condensation and morphology of model systems and to study the ability of BCE components to penetrate into the lipid environment. The in vitro tests were also done on different bacteria species (E. coli, Enterococcus faecalis, S. aureus, Salmonella enterica, Pseudomonas aeruginosa) to compare antimicrobial potency of the studied formulation. As evidenced the in vitro studies BCE formulation exerts very similar antibacterial activity against E. coli and S. aureus. Moreover, based on the collected data it is impossible to indicate which bacteria: Gram-positive or Gram-negative are more susceptible to this formulation. Model membrane experiments evidenced that the studied preservative affects organization of both E. coli and S. aureus model system by decreasing their condensation and altering their morphology. BCE components are able to penetrate into the lipid systems. However, all these effects depend on the lipid composition and monolayer organization. The collected results were analyzed from the point of view of the mechanism of action of blackcurrant extract and the factors, which may determine the activity of this formulation.
- Record ID
- CUTef3cbd17237644388b6bbb661799d26e
- Publication categories
- ;
- Author
- Journal series
- Archives of Biochemistry and Biophysics, ISSN 0003-9861, e-ISSN 1096-0384
- Issue year
- 2023
- Vol
- 750
- Pages
- [1-11]
- Article number
- 109806
- Other elements of collation
- fot.; tab.; wykr.; Bibliografia (na s.) - 10-11; Bibliografia (liczba pozycji) - 44; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 750
- Keywords in English
- blackcurrant extract, langmuir monolayers, bacteria model membranes, In vitro studies
- ASJC Classification
- ; ;
- DOI
- DOI:10.1016/j.abb.2023.109806 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/pii/S0003986123003053 Opening in a new tab
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTef3cbd17237644388b6bbb661799d26e/
- URN
urn:pkr-prod:CUTef3cbd17237644388b6bbb661799d26e
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.