Liposomes as carriers of GHK-Cu tripeptide for cosmetic application
Authors:
- Michał Dymek,
- Karolina Olechowska,
- Katarzyna Hąc-Wydro,
- Elżbieta Sikora
Abstract
first_page settings Order Article Reprints Open AccessArticle Liposomes as Carriers of GHK-Cu Tripeptide for Cosmetic Application by Michał Dymek 1 [ORCID] , Karolina Olechowska 2 [ORCID] , Katarzyna Hąc-Wydro 2 and Elżbieta Sikora 1,* [ORCID] 1 Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Kraków, Poland 2 Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland * Author to whom correspondence should be addressed. Pharmaceutics 2023, 15(10), 2485; https://doi.org/10.3390/pharmaceutics15102485 Received: 13 September 2023 / Revised: 13 October 2023 / Accepted: 16 October 2023 / Published: 18 October 2023 (This article belongs to the Special Issue Colloidal Nanocarriers for Dermatological Diseases Therapy) Download keyboard_arrow_down Browse Figures Versions Notes Abstract Liposomes are self-assembled spherical systems composed of amphiphilic phospholipids. They can be used as carriers of both hydrophobic and hydrophilic substances, such as the anti-aging and wound-healing copper-binding peptide, GHK-Cu (glycyl-L-histidyl-L-lysine). Anionic (AL) and cationic (CL) hydrogenated lecithin-based liposomes were obtained as GHK-Cu skin delivery systems using the thin-film hydration method combined with freeze–thaw cycles and the extrusion process. The influence of total lipid content, lipid composition and GHK-Cu concentration on the physicochemical properties of liposomes was studied. The lipid bilayer fluidity and the peptide encapsulation efficiency (EE) were also determined. Moreover, in vitro assays of tyrosinase and elastase inhibition were performed. Stable GHK-Cu-loaded liposome systems of small sizes (approx. 100 nm) were obtained. The bilayer fluidity was higher in the case of cationic liposomes. As the best carriers, 25 mg/cm3 CL and AL hydrated with 0.5 mg/cm3 GHK-Cu were selected with EE of 31.7 ± 0.9% and 20.0 ± 2.8%, respectively. The obtained results confirmed that the liposomes can be used as carriers for biomimetic peptides such as copper-binding peptide and that the GHK-Cu did not significantly affect the tyrosinase activity but led to 48.90 ± 2.50% elastase inhibition, thus reducing the rate of elastin degeneration and supporting the structural integrity of the skin.
- Record ID
- CUTd0e47ad9e4ea4f108a9a39922adcc2bd
- Publication categories
- ;
- Author
- Journal series
- Pharmaceutics, ISSN , e-ISSN 1999-4923, Monthly
- Issue year
- 2023
- Vol
- 15
- No
- 10
- Pages
- [1-17]
- Article number
- 2485
- Other elements of collation
- fot.; tab.; wykr.; Bibliografia (na s.) - 13-17; Bibliografia (liczba pozycji) - 92; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 15, Iss. 10
- Substantive notes
- This article belongs to the Special Issue: Colloidal Nanocarriers for Dermatological Diseases Therapy
- Keywords in English
- liposomes, copper tripeptide, encapsulation efficiency, cosmetics
- ASJC Classification
- DOI
- DOI:10.3390/pharmaceutics15102485 Opening in a new tab
- URL
- https://www.mdpi.com/1999-4923/15/10/2485 Opening in a new tab
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Score source
- journalList
- Score
- Publication indicators
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTd0e47ad9e4ea4f108a9a39922adcc2bd/
- URN
urn:pkr-prod:CUTd0e47ad9e4ea4f108a9a39922adcc2bd
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