Concentration of polycyclic aromatic hydrocarbons (PAHs) in human serum and adipose tissues and stimulatory effect of naphthalene in adipogenesis in 3T3-L1 cells
Authors:
- Ewa Mlyczyńska,
- Alice Bongrani,
- Christelle Rame,
- Małgorzata Węgiel,
- Anna Maślanka,
- Piotr Major,
- Piotr Zarzycki,
- Pierre-Henri Ducluzeau,
- Arnaud De Luca,
- Celine Bourbao-Tournois,
- Pascal Froment,
- Agnieszka Rak,
- Joëlle Dupont
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are one of the most prevalent classes of environmental pollutants. Some evidence shows that PAHs could be involved in human obesity. However, little is known about the distribution patterns of PAHs in human adipose tissue (AT) and the role of PAHs on adipogenesis/lipogenesis. The aims of this pilot study were to determine concentrations of 16 PAHs defined as high-priority pollutants in the plasma and adipose tissue of French and Polish bariatric patients, as well as their correlation with body mass index (BMI), plasma and AT adipokines expression levels. We finally investigated the role of naphthalene on cell proliferation, viability, and differentiation in 3T3-L1 preadipocytes. The concentration of most PAHs was similar in the three types of AT and it was significantly higher in AT as compared to plasma, suggesting bioaccumulation. Polish patients had higher PAH levels in AT than French ones. Only the concentration of naphthalene in AT was positively correlated with the BMI and serum or adipose chemerin, adiponectin and resistin expression, in French but not in Polish patients, who had significantly higher BMIs. Moreover, naphthalene exposure increased the cell proliferation of 3T3-L1 preadipocytes and lipogenesis, and increased the expression of genes involved in adipogenesis after cell differentiation. Taken together, PAHs and more particularly naphthalene could be an obesogenic molecule and increase the risk of obesity.
- Record ID
- CUT7affd95524ee4b09b7170ba60edff6f4
- Publication categories
- ;
- Author
- Journal series
- International Journal of Molecular Sciences, ISSN 1661-6596, e-ISSN 1422-0067, Biweekly
- Issue year
- 2023
- Vol
- 24
- No
- 2
- Pages
- [1-19]
- Article number
- 1455
- Other elements of collation
- fot.; tab.; wykr.; Bibliografia (na s.) - 16-19; Bibliografia (liczba pozycji) - 64; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 24, Iss. 2
- Substantive notes
- This article belongs to the Special Issue: Environmental Toxicant Exposures and Metabolic Disease
- Keywords in English
- polycyclic aromatic hydrocarbons, naphthalene, adipose tissue, 3T3-L1 cells, adipokines
- ASJC Classification
- ; ; ; ; ; ; ;
- DOI
- DOI:10.3390/ijms24021455 Opening in a new tab
- URL
- https://www.mdpi.com/1422-0067/24/2/1455 Opening in a new tab
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT7affd95524ee4b09b7170ba60edff6f4/
- URN
urn:pkr-prod:CUT7affd95524ee4b09b7170ba60edff6f4
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.