Poly(octamethylene citrate) modified with glutathione as a promising material for vascular tissue engineering
Authors:
- Agata Flis,
- Martina Trávníčková,
- Filip Koper,
- Karolina Knap,
- Wiktor Kasprzyk,
- Lucie Bačáková,
- Elżbieta Pamuła
Abstract
One of the major goals of vascular tissue engineering is to develop much-needed materials that are suitable for use in small-diameter vascular grafts. Poly(1,8-octamethylene citrate) can be considered for manufacturing small blood vessel substitutes, as recent studies have demonstrated that this material is cytocompatible with adipose tissue-derived stem cells (ASCs) and favors their adhesion and viability. The work presented here is focused on modifying this polymer with glutathione (GSH) in order to provide it with antioxidant properties, which are believed to reduce oxidative stress in blood vessels. Cross-linked poly(1,8-octamethylene citrate) (cPOC) was therefore prepared by polycondensation of citric acid and 1,8-octanediol at a 2:3 molar ratio of the reagents, followed by in-bulk modification with 0.4, 0.8, 4 or 8 wt.% of GSH and curing at 80 °C for 10 days. The chemical structure of the obtained samples was examined by FTIR-ATR spectroscopy, which confirmed the presence of GSH in the modified cPOC. The addition of GSH increased the water drop contact angle of the material surface and lowered the surface free energy values. The cytocompatibility of the modified cPOC was evaluated in direct contact with vascular smooth-muscle cells (VSMCs) and ASCs. The cell number, the cell spreading area and the cell aspect ratio were measured. The antioxidant potential of GSH-modified cPOC was measured by a free radical scavenging assay. The results of our investigation indicate the potential of cPOC modified with 0.4 and 0.8 wt.% of GSH to produce small-diameter blood vessels, as the material was found to: (i) have antioxidant properties, (ii) support VSMC and ASC viability and growth and (iii) provide an environment suitable for the initiation of cell differentiation.
- Record ID
- CUTae5053f57fa648b9b7bcb3e7287f08df
- Publication categories
- ;
- Author
- Journal series
- Polymers, ISSN , e-ISSN 2073-4360, Biweekly
- Issue year
- 2023
- Vol
- 15
- No
- 5
- Pages
- [1-16]
- Article number
- 1322
- Other elements of collation
- fot.; tab.; wykr.; Bibliografia (na s.) - 15-16; Bibliografia (liczba pozycji) - 37; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 15, Iss. 5
- Substantive notes
- This article belongs to the Special Issue: Polymer Scaffolds for Tissue Engineering II
- Keywords in English
- poly(alkylene citrates), poly(1,8-octametylene citrate), citric acid, vascular tissue engineering, glutathione (GSH), cytocompatibility, vascular smooth-muscle cells (VSMCs), adipose tissue-derived stem cells (ASCs)
- ASJC Classification
- ;
- DOI
- DOI:10.3390/polym15051322 Opening in a new tab
- URL
- https://www.mdpi.com/2073-4360/15/5/1322 Opening in a new tab
- Related project
- Nowe fluorescencyjne poliestry o właściwościach przeciwutleniających do zastosowań w inżynierii naczyń krwionośnych. . Project leader at PK: , ,
Projects financed by NSC [Projekty finansowane przez NCN] - Language
- eng (en) English
- License
- Score (nominal)
- 100
- Score source
- journalList
- Score
- Publication indicators
- Citation count
- 4
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTae5053f57fa648b9b7bcb3e7287f08df/
- URN
urn:pkr-prod:CUTae5053f57fa648b9b7bcb3e7287f08df
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.