New coumarin derivatives as potential fluorescent probes for bioimaging applications
Authors:
- Patrycja Środa
Abstract
Fluorescence microscopy of live cells is an integral part of modern cell biology. It is based on the registration of light emitted by fluorophore molecules excited with the appropriate wavelength. Bio-imaging relates to methods that non-invasively visualize biological processes in real-time, and it aims to interfere as little as possible with life processes. Moreover, it is often used to gain information on the observed specimen's 3-D structure from the outside, i.e., without physical interference. Fluorescence microscopy allows for labeling interesting cell elements (e.g., proteins or organelles) with fluorophores with given properties. Fluorescent probes (fluorophores) are based on small organic molecules that provide dynamic information concerning the localization and quantity of the molecules of interest without the need for genetic engineering of the sample. New coumarin derivatives played the role of fluorophores in the research. The research scope included assessing the possibility of penetration of the analyzed 3-(2-pyridyl)coumarin derivatives as fluorescent probes into human non-small cell lung cancer cells. During research line of non-small-cell lung carcinoma (A549) have been used. The test compounds showed the ability to penetrate the cells and emit fluorescence. A very desirable property of fluorescent probes is their safety, i.e., no toxic effect on cells. Therefore, the MTT test was performed as part of the research. It enabled the evaluation of the cytotoxicity of the analyzed 3-(2-pyridyl)coumarin derivatives. It has been shown that twelve out of fifteen 3-(2-pyridyl)coumarin derivatives can penetrate biological membranes of cells and show fluorescence emission. Positive results of the MTT test were obtained. They prove the safety of using 3-(2-pyridyl)coumarin derivatives as molecular fluorescent probes of eukaryotic cells in fluorescence microscopy.
- Record ID
- CUTd3a50c57ac9343da9fb68f67ed4417ac
- Publication categories
- ; ;
- Author
- Pages
- 52-53
- Book
- Drabik Sylwia, Sylwia Drabik Rauk Zuzanna, Zuzanna Rauk Wójcikowska Olga Olga Wójcikowska (eds.): 6th International Conference of Cell Biology : book of abstracts, 2020, Kraków, [Jagiellonian University]
- Keywords in English
- bioimaging, eukaryotic cells, fluorescent probes, fluorescence microscopy
- Related project
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Language
- eng (en) English
- Score (nominal)
- 0
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTd3a50c57ac9343da9fb68f67ed4417ac/
- URN
urn:pkr-prod:CUTd3a50c57ac9343da9fb68f67ed4417ac
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.