Colocalization of new coumarin-based fluorescent probes using LysoTracker Red and MitoTracker Red probes
Authors:
- Patrycja Środa,
- Joanna Ortyl,
- Paulina Koczurkiewicz-Adamczyk
Abstract
Fluorescence bioimaging is an excellent tool for analyzing internal cellular structures and observing dynamic processes occurring in eukaryotic cells using fluorescent dyes. Desirable characteristics of fluorescent dyes include the ability to penetrate into cells, high fluorescence efficiency and selectivity in the staining process. This enables clear, unambiguous visualization and location of structures inside the cells. In addition, these compounds should not be cytotoxic in order to be used safely on cells. Coumarin derivatives were examined spectroscopically for use in imaging of eukaryotic cells in fluorescence microscopy. A non-small cell lung cancer line (A549) was used during the study. Analysis performed with the Leica DMiL LED Fluo inverted microscope. These compounds have been shown to be effective as fluorescent dyes. LysoTracker and MitoTracker Red are fluorescent probes widely used for viable cell staining of lysosomes and mitochondria, respectively. The molecular structures of LysoTracker Red (Figure A) and MitoTracker Red (Figure B). These commercial probes were used in colocalization studies of novel coumarin derivatives. The partial localization of new coumarin derivatives in lysosomes was confirmed. In addition, the effect of coumarin derivative compounds on the cytotoxicity of cells was checked. For this purpose, a colorimetric MTT test was used. Cytotoxicity of the tested compounds was compared with the doxorubicin compound, which has proven toxicity to cancer cells. After the MTT test, it was shown that the coumarin derivatives tested are safe for cells and do not affect their physiological properties after both 3 and 24 hours of incubation.
- Record ID
- CUT454f3c658cfc49a685c5a8aa5b7f3f95
- Publication categories
- ;
- Author
- Pages
- 126
- Other elements of collation
- rys.
- Substantive notes
- Data wyd. wg daty konf.
- Book
- Filipiak Marcin Szymon, Marcin Szymon Filipiak Krupiński Piotr Piotr Krupiński (eds.): The 9th Prof. Krzysztof W. Szewczyk Intercollegiate Biotechnology Symposium SYMBIOZA, the 21st-23rd of May 2021, online : book of abstracts, 2021, Warsaw, Faculty of Biology. University of Warsaw, ISBN 978-83-954288-2-1
- Keywords in English
- fluorescence bioimaging, fluorescent dyes, colocalization, eukaryotic cells, fluorescence microscopy
- Related project
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Language
- eng (en) English
- Score (nominal)
- 0
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT454f3c658cfc49a685c5a8aa5b7f3f95/
- URN
urn:pkr-prod:CUT454f3c658cfc49a685c5a8aa5b7f3f95
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