Toxicity assessment and visualization of cell structures using new fluorophores from the group of coumarin derivatives
Authors:
- P. Środa,
- J. Ortyl,
- P. Koczurkiewicz-Adamczyk
Abstract
Fluorescent probes based on small organic molecules have become indispensable tools in modern biology because they provide dynamic information concerning the localization and quantity of the molecules of interest, without the need of genetic engineering of the sample. Moreover, fluorescent probes offer great potential to identify and treat surgical tumors by clinicians. For any biomedical applications, it is mandatory to investigate the cytotoxicity of fluorescent probes prior to their utilization into biological applications. This study evaluated the cytotoxicity of novel coumarin-derived fluorophores using the MTT (3- (4,5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide) cell viability assay on a non-small cell lung cancer line (A549). The test measures the metabolic activity of cells incubated in the presence of test compounds. The test used the ability of the enzyme mitochondrial dehydrogenase to reduce the tetrazolium salt to the colored formazan. The amount of formazan formed is directly proportional to the number of viable, actively proliferating cells in culture. Cytotoxicity of the tested compounds was compared with the doxorubicin compound, which has proven toxicity to cancer cells. After the MTT test, it was shown that the coumarin derivatives tested are safe for cells and do not affect their physiological properties after both 3 and 24 hours of incubation. The aim of this study was also to evaluate the ability to penetrate biological membranes and visualize cellular structures using novel compounds based on coumarin scaffolds. Analysis performed with the Leica DMiL LED Fluo inverted microscope. These compounds have been shown to be effective as fluorescent dyes. Colocalization studies of new coumarin derivatives were also performed using commercial probes LysoTracker and MitoTracker Red. The partial localization of new coumarin derivatives in lysosomes was confirmed.
- Record ID
- CUT861ed0b0665e475394de4ee87deff19d
- Publication categories
- ;
- Author
- Pages
- 106
- Substantive notes
- Data wyd. wg daty konf.
- Book
- 4th Central European Biomedical Congress, 7-9 June 2021 : abstract book, 2021, [S.l.], [s.n.], ISBN 978-83-942760-8-9
- Keywords in English
- fluorophores, visualization of cell structures, toxicity assessment
- URL
- https://cebc2021.fluidagency.pl/cebc-2021/ Opening in a new tab
- Related project
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Language
- eng (en) English
- License
- Score (nominal)
- 0
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT861ed0b0665e475394de4ee87deff19d/
- URN
urn:pkr-prod:CUT861ed0b0665e475394de4ee87deff19d
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