Development of novel fluorescent probes to detect and quantify specific reactive oxygen species
Authors:
- Patryk Szymaszek,
- Patrycja Środa,
- Małgorzata Tyszka-Czochara,
- Anna Chachaj-Brekiesz,
- Tomasz Świergosz,
- Joanna Ortyl
Abstract
Hydrogen peroxide (H2O2) plays important roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the synthesis of a novel fluorescent probe based on pyridine-coumarin core 3-(2-pyridyl)-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)c hromen-2-one (P182) for quantitative measurement of H2O2. This fluorometric probe displays a fluorescence turn-on response in the process of deprotection of arylboronate to phenol in the presence of H2O2. It could offer good performances in terms of sensitivity and response time. Moreover, cytotoxicity studies on various model cell lines proved the non-toxic activity of the tested sensor allowing its use in vivo studies. This study provides research on molecular fluorescence probe for the detection of H2O2.
- Record ID
- CUT71dcd4c209d9424abba046af13f3eba1
- Publication categories
- ;
- Author
- Journal series
- Journal of Molecular Liquids, ISSN 0167-7322, e-ISSN 1873-3166
- Issue year
- 2023
- Vol
- 369
- Pages
- [1-10]
- Article number
- 120884
- Other elements of collation
- schem.; tab.; wykr.; Bibliografia (na s.) - 8-10; Bibliografia (liczba pozycji) - 83; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 369
- Substantive notes
- Appendix A. Supplementary data can be found online at: https://doi.org/10.1016/j.molliq.2022.120884
- Keywords in English
- fluorescence, probe, reactive oxygen species (ROS), cytotoxicity
- ASJC Classification
- ; ; ; ; ;
- DOI
- DOI:10.1016/j.molliq.2022.120884 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/pii/S0167732222024230 Opening in a new tab
- Related project
- Diamentowy Grant "Projektowanie i synteza nowych sensorów luminescencyjnych oraz ich fotochemiczne badania potencjalnej przydatności do selektywnego i wielofunkcyjnego zastosowania w diagnostyce molekularnej. . Project leader at PK: , ,
- Language
- eng (en) English
- Score (nominal)
- 100
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT71dcd4c209d9424abba046af13f3eba1/
- URN
urn:pkr-prod:CUT71dcd4c209d9424abba046af13f3eba1
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.