Synergistic antifungal interactions between antibiotic amphotericin B and selected 1,3,4-thiadiazole derivatives, determined by microbiological, cytochemical, and molecular spectroscopic studies
Authors:
- Agnieszka Dróżdż,
- Dominika Kubera,
- Adrianna Sławińska-Brych,
- Arkadiusz Matwijczuk,
- Lidia Ślusarczyk,
- Grzegorz Czernel,
- Dariusz Karcz,
- Alina Olender,
- Agnieszka Bogut,
- Daniel Pietrzak,
- Wojciech Dąbrowski,
- Andrzej Stepulak,
- Alicja Wójcik-Załuska,
- Mariusz Gagoś
Abstract
In recent years, drug-resistant and multidrug-resistant fungal strains have been more frequently isolated in clinical practice. This phenomenon is responsible for difficulties in the treatment of infections. Therefore, the development of new antifungal drugs is an extremely important challenge. Combinations of selected 1,3,4-thiadiazole derivatives with amphotericin B showing strong synergic antifungal interactions are promising candidates for such formulas. In the study, microbiological, cytochemical, and molecular spectroscopy methods were used to investigate the antifungal synergy mechanisms associated with the aforementioned combinations. The present results indicate that two derivatives, i.e., C1 and NTBD, demonstrate strong synergistic interactions with AmB against some Candida species. The ATR-FTIR analysis showed that yeasts treated with the C1 + AmB and NTBD + AmB compositions, compared with those treated with single compounds, exhibited more pronounced abnormalities in the biomolecular content, suggesting that the main mechanism of the synergistic antifungal activity of the compounds is related to a disturbance in cell wall integrity. The analysis of the electron absorption and fluorescence spectra revealed that the biophysical mechanism underlying the observed synergy is associated with disaggregation of AmB molecules induced by the 1,3,4-thiadiazole derivatives. Such observations suggest the possibility of the successful application of thiadiazole derivatives combined with AmB in the therapy of fungal infections.
- Record ID
- CUT14a5cb6547b44873926e17018cca1dcc
- Publication categories
- ;
- Author
- Journal series
- International Journal of Molecular Sciences, ISSN 1661-6596, e-ISSN 1422-0067, Biweekly
- Issue year
- 2023
- Vol
- 24
- No
- 4
- Pages
- [1-26]
- Article number
- 3430
- Other elements of collation
- fot.; rys.; tab.; wykr.; Bibliografia (na s.) - 23-26; Bibliografia (liczba pozycji) - 68; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 24, Iss. 4
- Substantive notes
- This article belongs to the Special Issue: Synergistic Properties of Selected Compounds with Antibiotics—Spectroscopic and Biological Studies
- Keywords in English
- amphotericin B, 1,3,4-thiadiazole derivatives, synergy mechanisms, FTIR spectroscopy, molecular spectroscopy, molecular aggregation
- ASJC Classification
- ; ; ; ; ; ; ;
- DOI
- DOI:10.3390/ijms24043430 Opening in a new tab
- URL
- https://www.mdpi.com/1422-0067/24/4/3430 Opening in a new tab
- Related project
- Badanie skuteczności i mechanizmów grzybobójczego działania wybranych pochodnych 1,3,4-tiadiazoli w interakcji z antybiotykami z grupy azoli i polienów wobec szczepów wykazujących oporność specyficzną lub wielolekową. . Project leader at PK: , ,
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT14a5cb6547b44873926e17018cca1dcc/
- URN
urn:pkr-prod:CUT14a5cb6547b44873926e17018cca1dcc
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.