Development of chitosan/gelatin-based hydrogels incorporated with albumin particles
Authors:
- Magdalena Bańkosz
Abstract
The research subject of this paper are natural polymer-based hydrogels modified with albumin particles. The proteins were obtained via the salt-induced precipitation method, and next characterized using dynamic light scattering (DLS), UV-Vis spectroscopy and FT-IR spectroscopy. The most favorable composition showing monodispersity and particles with a size lower than 40 nm was selected for modification of hydrogels. Such systems were obtained via the photopolymerization performed under the influence of UV radiation using diacrylate poly(ethylene glycol) as a crosslinking agent and 2-hydroxy-2-methylpropiophenone as a photoinitiator. Next, the hydrogels’ swelling ability, mechanical properties, wettability and surface morphology were characterized. Moreover, FT-IR spectroscopy, incubation studies in simulated physiological liquids, pro-inflammatory activity analysis and MTT reduction assay with L929 murine fibroblasts were performed. The release profiles of proteins from hydrogels were also verified. Materials modified with proteins showed higher swelling ability, increased flexibility even by 50% and increased surface hydrophilicity. Hydrogels’ contact angles were within the range 62–69◦ while the tensile strength of albumin-containing hydrogels was approx. 0.11 MPa. Furthermore, the possibility of the effective release of protein particles from hydrogels in acidic environment (approximately 70%) was determined. Incubation studies showed hydrogels’ stability and lack of their degradation in tested media. The viability of fibroblasts was 89.54% for unmodified hydrogel, and approx. 92.73% for albumin-modified hydrogel, and such an increase indicated the positive impact of the albumin on murine fibroblast proliferation.
- Record ID
- CUT48c6c9c685584806a15ee6b561356f51
- Publication categories
- ;
- Author
- Journal series
- International Journal of Molecular Sciences, ISSN 1661-6596, e-ISSN 1422-0067, Biweekly
- Issue year
- 2022
- Vol
- 23
- No
- 22
- Pages
- 1-21
- Article number
- 14136
- Other elements of collation
- rys.; tab.; wykr.; Bibliografia (na s.) - 19-21; Bibliografia (liczba pozycji) - 56; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 23, Iss. 22, Spec. Iss.
- Substantive notes
- Special Issue: Design and Synthesis of Biomedical Polymer Materials
- Keywords in English
- hydrogel materials, albumin carriers, swelling properties, release of protein, wound healing
- ASJC Classification
- ; ; ; ; ; ; ;
- DOI
- DOI:10.3390/ijms232214136 Opening in a new tab
- URL
- https://www.mdpi.com/1422-0067/23/22/14136 Opening in a new tab
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT48c6c9c685584806a15ee6b561356f51/
- URN
urn:pkr-prod:CUT48c6c9c685584806a15ee6b561356f51
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