Evaluation of the impact of pH of the reaction mixture, type of the stirring, and the reagents' concentration in the wet precipitation method on physicochemical properties of hydroxyapatite so as to enhance its biomedical application potential
Authors:
- Magdalena Głąb,
- Sonia Kudłacik-Kramarczyk,
- Anna Drabczyk,
- Aleksandra Kordyka,
- Marcin Godzierz,
- Paweł S. Wróbel,
- Agnieszka Tomala,
- Bożena Tyliszczak,
- Agnieszka Sobczak-Kupiec
Abstract
Hydroxyapatite (HAp) constitutes a significant inorganic compound which due to its osteoinductivity, osteoconductivity as well as the ability to promote bone growth and regeneration is widely applied in development of biomaterials designed for bone tissue engineering. In this work, various synthesis methodologies of HAp based on the wet precipitation technique were applied, and the impact of pH of the reaction mixture, the concentration of individual reagents as well as the type of stirring applied (mechanical/magnetic) on the properties of final powders was discussed. Spectroscopic methods (Fourier transform infrared, Raman) and X-ray diffraction allowed to verify the synthesis parameters leading to obtaining calcium phosphate with 96% HAp in phase which indicated higher homogeneity of obtained powder (93.4%) than commercial HAp. Powders' morphology was evaluated using microscopic techniques while specific surface area was determined via Brunauer–Emmett– Teller analysis. Particle size distribution, porosity of powders, and stability of HAp suspensions were also characterized. It was proved that synthesis at pH = 11.0 using mechanical stirring resulted in calcium phosphate with a high phase homogeneity and homogeneous pore size distribution (6–20 nm). Moreover, obtained HAp powder showed 71.8% more specific surface area than commercial material—that is, 110 m3/g for synthetic HAp and 64 m3/g in the case of commercial powder—which, in turn, is significant in terms of its potential application as carrier of active substances. Thus it was demonstrated that by applying appropriate conditions of HAp synthesis it is possible to obtain powder with properties enhancing its application potential for medical purposes.
- Record ID
- CUT03d5631d870e41ffa7f73539735b67f7
- Publication categories
- ;
- Author
- Journal series
- Journal of Biomedical Materials Research Part B-Applied Biomaterials, ISSN 1552-4973, e-ISSN 1552-4981
- Issue year
- 2022
- Vol
- 110
- No
- 12
- Pages
- 2649-2666
- Other elements of collation
- rys.; tab.; wykr.; Bibliografia (na s.) - 2665-2666; Bibliografia (liczba pozycji) - 41; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 110, Iss. 12
- Keywords in English
- adsorption-desorption isotherms, hydroxyapatite, pore size distribution, sedimentation rate, specific surface area, suspension stability, wet precipitation method
- ASJC Classification
- ;
- DOI
- DOI:10.1002/jbm.b.35118 Opening in a new tab
- URL
- https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.b.35118 Opening in a new tab
- Related project
- Wielofunkcyjne kompozyty aktywne biologicznie do zastosowań w medycynie regeneracyjnej układu kostnego. . Project leader at PK: , ,
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT03d5631d870e41ffa7f73539735b67f7/
- URN
urn:pkr-prod:CUT03d5631d870e41ffa7f73539735b67f7
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.