Pyridine derivatives as candidates for selective and sensitive fluorescent biosensors for lung cancer cell imaging and iron ions detection
Authors:
- Monika Topa-Skwarczyńska,
- Patryk Szymaszek,
- Paweł Fiedor,
- Anna Chachaj-Brekiesz,
- Mariusz Galek,
- Wiktor Kasprzyk,
- Paulina Koczurkiewicz-Adamczyk,
- Filip Petko,
- Elżbieta Pękala,
- Malgorzata Tyszka-Czochara,
- Dariusz Bogdał,
- Tomasz Świergosz,
- Roman Popielarz,
- Joanna Ortyl
Abstract
A series of 2-amino-4,6-diphenyl-pyridine-3-carbonitrile (APC) derivatives has been synthesized and their applicability for the role of fluorescent molecular sensors for chemical and biochemical applications has been evaluated. It has been found that the dimethylamino-substituted APC derivatives exhibit a very high solvatochromic effect, that makes them good candidates for application as chemosensors of polarity changes. The fluorescence intensity and emission wavelength of some of the derivatives turned out to be very sensitive to trace amounts of Hg2+, Hg22+, Sb3+ and Bi3+ ions, while other derivatives are selectively sensitive to the presence of Fe2+ and Fe3+ ions. Hence, these derivatives can be applied for the determination of those ions concentration in various media, such as waste water, food products or contaminated soils. Moreover, the applicability of the sensors studied for living lung cancer cells imaging was assessed. It has been found that all of the APC derivatives show good photostability under irradiation conditions used in fluorescence microscopy, while their response is not affected significantly by changes in temperature. Additionally, MTT assays indicated that these sensors were safe for the living cells. So, some of the derivatives studied may be applied for fluorescent staining of the living cells to enable their visualization by fluorescence microscopy.
- Record ID
- CUT24d5ee37328f4c17b6bd14707a8cb150
- Publication categories
- ;
- Author
- Journal series
- Dyes and Pigments, ISSN 0143-7208, e-ISSN 1873-3743
- Issue year
- 2022
- Vol
- 200
- Pages
- [1-14]
- Article number
- 110171
- Other elements of collation
- fot.; rys.; tab.; wykr.; Bibliografia (na s.) - 13-14; Bibliografia (liczba pozycji) - 57; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 200
- Keywords in English
- pyridine derivatives, selective and sensitive fluorescent biosensors, lung cancer, cell imaging, iron ions, detection
- ASJC Classification
- ;
- DOI
- DOI:10.1016/j.dyepig.2022.110171 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/pii/S0143720822000936 Opening in a new tab
- Related project
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Diamentowy Grant "Projektowanie i synteza nowych sensorów luminescencyjnych oraz ich fotochemiczne badania potencjalnej przydatności do selektywnego i wielofunkcyjnego zastosowania w diagnostyce molekularnej. . Project leader at PK: , ,
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Language
- eng (en) English
- Score (nominal)
- 100
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT24d5ee37328f4c17b6bd14707a8cb150/
- URN
urn:pkr-prod:CUT24d5ee37328f4c17b6bd14707a8cb150
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.