Molecular interactions of bovine serum albumin (BSA) with pyridine derivatives as candidates for non-covalent protein probes: a spectroscopic investigation
Authors:
- Patryk Szymaszek,
- Paweł Fiedor,
- Anna Chachaj-Brekiesz,
- Małgorzata Tyszka-Czochara,
- Tomasz Świergosz,
- Joanna Ortyl
Abstract
Research regarding protein-ligand interactions remains a major concern in the fields of chemistry, biochemistry, pharmacology, and food science. Herein, the interaction of selected pyridine derivatives with bovine serum albumin (BSA) was investigated using UV–Vis and fluorescence spectroscopy. A series of 2-amino-4,6-diphenyl-pyridinium-3-carbonitrile derivatives as fluorescent probes for the determination of BSA revealed that the probes investigated lead to a 12-176-fold increase in emission intensity. The calculated binding parameters support the conclusion that probes bind to BSA in 1:1 stoichiometry with moderate strength, and the quenching for pyridine probes is due to static interaction. The calculated negative value of the free energy change indicates that the complexation is a spontaneous process. Furthermore, from the estimated entropy and enthalpy change values, complexation between the pyridine derivatives and BSA was found to occur primarily via van der Waals interaction and hydrogen bonding. The pH-dependent emission experiment showed that BSA detection with the evaluated probes could be conducted at physiological pH = 7.4 with a wide safety range.
- Record ID
- CUT959eaff3c3fb4f26b33cc9836ebb7d8a
- Publication categories
- ;
- Author
- Journal series
- Journal of Molecular Liquids, ISSN 0167-7322, e-ISSN 1873-3166
- Issue year
- 2022
- Vol
- 347
- Pages
- [1-12]
- Article number
- 118262
- Other elements of collation
- tab.; Bibliografia (na s.) - 11-12; Bibliografia (liczba pozycji) - 72; Oznaczenie streszczenia - Abstr.; Data udostępnienia on-line - 2021-12-07; Numeracja w czasopiśmie - Vol. 347
- Keywords in English
- proteins, BSA, Fluorescence probes, spectroscopy, molecular interactions
- ASJC Classification
- ; ; ; ; ;
- DOI
- DOI:10.1016/j.molliq.2021.118262 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/pii/S0167732221029871 Opening in a new tab
- Related project
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Diamentowy Grant "Projektowanie i synteza nowych sensorów luminescencyjnych oraz ich fotochemiczne badania potencjalnej przydatności do selektywnego i wielofunkcyjnego zastosowania w diagnostyce molekularnej. . Project leader at PK: , ,
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Language
- eng (en) English
- Score (nominal)
- 100
- Score source
- journalList
- Score
- Publication indicators
- Additional fields
- Indeksowana w: Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT959eaff3c3fb4f26b33cc9836ebb7d8a/
- URN
urn:pkr-prod:CUT959eaff3c3fb4f26b33cc9836ebb7d8a
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.