Investigation on green synthesis, biocompatibility, and antibacterial activity of silver nanoparticles prepared using Cistus incanus
Authors:
- Wioletta Florkiewicz,
- Klaudia Pluta,
- Dagmara Malina,
- Karolina Rudnicka,
- Anna Żywicka,
- Martin Duarte Guigou,
- Bożena Tyliszczak,
- Agnieszka Sobczak-Kupiec
Abstract
This paper describes the plant-mediated preparation of silver nanoparticles with aqueous extract and infusion of Cistus incanus leaves. To evaluate aqueous extract and infusion antioxidant capacity and total phenolic content the DPPH and Folin–Ciocalteau methods were utilized. The antioxidant capacity and total phenolic content of extract and infusion were equal to 85.97 ± 6.54 mg gallic acid equivalents per gram of dry weight.; 10.76 ± 0.59 mg/mL and 12.65 ± 1.04 mg gallic acid equivalents per gram of dry weight.; 3.10 ± 0.14 mg/mL, respectively. The formed nanoparticles displayed the characteristic absorption band in the 380–450 nm wavelength range. The average size of particles was in the 68.8–71.2 nm range. Morphology and phase composition analysis revealed the formation of spherical nanoparticles with a face-centred cubic structure. Immune compatibility tests of nanoparticles and plant extracts showed no activation of the THP1-XBlue™ monocyte. Cytotoxicity tests performed with L929 mice fibroblasts showed that nanoparticles should be utilized at a concentration of 16 ppm. The minimum inhibitory concentrations determined with the microdilution method for nanoparticles prepared with plant infusion for S. aureus and S. epidermidis were 2 ppm and 16 ppm, respectively.
- Record ID
- CUTf46a042988464b54b4ff9dcc1fc83b35
- Publication categories
- ;
- Author
- Journal series
- Materials, ISSN , e-ISSN 1996-1944, Biweekly
- Issue year
- 2021
- Vol
- 14
- No
- 17
- Pages
- [1-18]
- Article number
- 5028
- Other elements of collation
- fot.; tab.; wykr.; Bibliografia (na s.) - 15-18; Bibliografia (liczba pozycji) - 68; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 14, Iss. 17
- Keywords in English
- silver nanoparticles, green synthesis, biocompatibility, antibacterial efficacy
- DOI
- DOI:10.3390/ma14175028 Opening in a new tab
- URL
- https://www.mdpi.com/1996-1944/14/17/5028#metrics Opening in a new tab
- Related project
- Opracowanie składu powłok kompozytowych modyfikowanych nanocząstkami metalicznymi zawierających fosforany wapnia pochodzenia naturalnego i syntetycznego. . Project leader at PK: , ,
Projects financed by NSC [Projekty finansowane przez NCN] - E-mobilność oraz zrównoważone materiały i technologie. . Project leader at PK: , ,
- Opracowanie składu powłok kompozytowych modyfikowanych nanocząstkami metalicznymi zawierających fosforany wapnia pochodzenia naturalnego i syntetycznego. . Project leader at PK: , ,
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Publication indicators
- = 6
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTf46a042988464b54b4ff9dcc1fc83b35/
- URN
urn:pkr-prod:CUTf46a042988464b54b4ff9dcc1fc83b35
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.