Spectrophotometric characterisation and research of the usefulness of potential highly selective luminescent sensors for the determination of metal ions
Authors:
- Patryk Szymaszek,
- Paweł Fiedor,
- Joanna Ortyl,
- Anna Chachaj-Brekiesz
Abstract
The development of analytical tools for the identification and quantification of metal ions is of great interest to scientists dealing with environmental protection, medicine or cell biology. Metal ions are essential for the proper functioning of a single cell and the whole organism. For this reason, visualization of the location and degree of oxidation will allow to determine their biological role and to understand the disorders and diseases caused by the disturbance of specific ions. The development of new fluorescent sensors focuses mainly on obtaining probes that will be highly selective and specific for one particular type of ion. Several methods of iron ion detection such as absorption atomic spectroscopy, voltamperometry or colorimetry are known, but fluorimetry is the most popular of these methods. The use of fluorimetry in combination with a fluorescent chemosensor allows the determination of metal ions functions in living organisms. By combining it with microscopic imaging, fluorescent sensors can be a powerful tool for determining the presence and concentration of ions. Although there are many sensors available that interact specifically with ions, the photostability of sensors is an uncut problem. In addition, sensors specific to such paramagnetic ions as iron ions have low selectivity in the presence of other paramagnetic ions such as Cu2+, Ni2+. For this reason it is important to develop high selectivity sensors with one of them. Bearing in mind the need to develop new selective fluorescence sensors, new derivatives of 2-amino-4,6-diphenylpyridine-3-carbonitrile were tested as fluorescence probes. The influence of the formation of inclusion complexes of the tested sensors with cyclodextrins on their solubility was also tested. Sensor sensitivity was tested using Infinite 200 PRO NanoQuant multi-level microplate reader by Tecan. The measurement consisted in recording the fluorescence spectrum of samples located in individual wells of the plate at a given wavelength.
- Record ID
- CUTc4ea3ae7ce5546c995bbb986a68ee81b
- Publication categories
- ;
- Author
- Pages
- 95
- Substantive notes
- Data wyd. wg daty konf.
- Book
- International Young Scientists Conference on Molecular and Cell Biology, February 25th -26th, 2021, Warsaw : abstract book, 2021, [S.l.], [s.n.]
- Keywords in English
- probe, sensor, luminescence, fluorescence, metal ion, cyclodextrin
- URL
- https://www.iimcb.gov.pl/en/young-scientists-conference Opening in a new tab
- Related project
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Language
- eng (en) English
- License
- Score (nominal)
- 0
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTc4ea3ae7ce5546c995bbb986a68ee81b/
- URN
urn:pkr-prod:CUTc4ea3ae7ce5546c995bbb986a68ee81b
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