Research on the usefulness of new non-covalent fluorescent sensors for the detection of biomacromolecules in medical diagnostics
Authors:
- Patryk Szymaszek
Abstract
Biosensors are analytical devices with high selectivity, which are increasingly used in medicine, environmental protection and food industry. In medicine, these sensors are used for diagnostic purposes, in environmental protection and in the food industry are used to detect harmful, toxic substances that can cause a genotoxic effect. There are many types of biosensors. In the case of protein biosensors, spectrofluorimetric measurements based on the measurement of light resulting from the interaction of the analyte with the sensor are used. The selection of an appropriate fluorescence technique for biosensor testing depends on the nature of the biosensor and its spectral properties. To characterize information signaled by a biosensor or an optical sensor based on the fluorescence phenomenon, several techniques are available: change of fluorescence intensity, change of fluorescence intensity, FRET (Förster resonance energy transfer), FLIM (Fluorescence life time imaging), FCS (Fluorescence correlation spectroscopy). The present research focuses on the technique of changing the fluorescence intensity and FRET. As part of this work, research was carried out on the non-covalent interaction of new fluorescent sensors that are derivatives of the coumarin with biomolecules, which is closely related to searches focused on the development of efficient spectroscopic probes dedicated to biochemical systems. These studies included, among other things, determining the effect of bovine serum albumin (BSA) concentration on the fluorescence of the test compounds, as well as the association of the coumarin sensor with BSA, and the changes in fluorescence over time. Due to the significant changes in the spectroscopic properties of the coumarin derivatives tested under the influence of the combination with the substance analyzed, which was BSA, it was shown that the analyzed compounds may find practical use as fluorescent markers for the determination of biomacromolecules.
- Record ID
- CUTcc7fa33c515b465493334e0822d0d902
- Publication categories
- ; ;
- Author
- Pages
- 72
- Substantive notes
- Wydaw. wg org. konf.
- Book
- Śmieszek Aleksandra Aleksandra Śmieszek (eds.): 5th International Conference of Cell Biology : book of abstracts, 2019, Kraków, Jagiellonian University
- Keywords in English
- fluorescent sensors, BSA, spectroscopy
- Related project
- Synthesis and photochemistry/photophysics studies of the intelligent luminescent molecular sensors for selective detection in biochemistry and chemistry. . Project leader at PK: , ,
- Language
- eng (en) English
- Score (nominal)
- 0
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTcc7fa33c515b465493334e0822d0d902/
- URN
urn:pkr-prod:CUTcc7fa33c515b465493334e0822d0d902
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