Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements
Authors:
- Wiktor Kasprzyk,
- Filip Koper,
- Agata Flis,
- Dominika Szreder,
- Elżbieta Pamuła,
- Dariusz Bogdał,
- Sławomir Wybraniec,
- Joanna Ortyl,
- Tomasz Świergosz
Abstract
Herein, a novel fluorescent method for the determination of GSH levels in aqueous solutions involving the utilization of citric acid as a derivatization reagent was developed. Therefore, the crucial parameters of the derivatization process were established from what has resulted in the development of a sensitive, reproducible, and accurate GSH assay. The method was validated, and its applicability in the characterization of the GSH concentration in dietary supplements concerning the selectivity in the determination of GSH over GSSG was both confirmed. The chemical structure of the new fluorophore 3-[(carboxymethyl)carbamoyl]-5-oxo-2,3-dihydro-5H-[1,3]thiazolo[3,2-a]pyridine-7-carboxylic acid – CTPC was elucidated using detailed NMR: one-dimensional (1H, 13C), as well as two-dimensional NMR spectra (1H–1H COSY, 1H–13C HSQC, 1H–13C HMBC, 1H–15N HSQC, 1H–15N HMBC) experiments. Besides, the essential optical, biological and antioxidative properties of CTPC were investigated.
- Record ID
- CUT2f469d7e702043b9b6f00410cd624df2
- Publication categories
- ;
- Author
- Journal series
- Analyst, ISSN 0003-2654, e-ISSN 1364-5528
- Issue year
- 2021
- Vol
- 146
- No
- 6
- Pages
- 1897-1906
- Other elements of collation
- rys.; tab.; wykr.; Bibliografia (na s.) - 1905-1906; Bibliografia (liczba pozycji) - 50; Oznaczenie streszczenia - Streszcz. ang.; Data udostępnienia on-line - 2021-01-08; Numeracja w czasopiśmie - Vol. 146, Iss. 6
- Keywords in English
- fluorescence assay, glutathione
- DOI
- DOI:10.1039/D0AN02245D Opening in a new tab
- URL
- https://pubs.rsc.org/en/content/articlelanding/2021/AN/D0AN02245D#!divAbstract Opening in a new tab
- Related project
- Nowe fluorescencyjne poliestry o właściwościach przeciwutleniających do zastosowań w inżynierii naczyń krwionośnych. . Project leader at PK: , ,
Projects financed by NSC [Projekty finansowane przez NCN] - Language
- eng (en) English
- Score (nominal)
- 100
- Publication indicators
- = 9
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT2f469d7e702043b9b6f00410cd624df2/
- URN
urn:pkr-prod:CUT2f469d7e702043b9b6f00410cd624df2
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.