The potential of novel chitosan-based scaffolds in pelvic organ prolapse (POP) treatment through tissue engineering
Authors:
- Julia Radwan-Pragłowska,
- Klaudia Stangel-Wójcikiewicz,
- Marek Piątkowski,
- Łukasz Janus,
- Dalibor Matýsek,
- Marcin Majka,
- Dalia Amrom
Abstract
The growing number of female reproductive system disorders creates a need for novel treatment methods. Tissue engineering brings hope for patients, which enables damaged tissue reconstruction. For this purpose, epithelial cells are cultured on three-dimensional scaffolds. One of the most promising materials is chitosan, which is known for its biocompatibility and biodegradability. The aim of the following study was to verify the potential of chitosan-based biomaterials for pelvic organ prolapse regeneration. The scaffolds were obtained under microwave-assisted conditions in crosslinking reactions, using dicarboxylic acids and aminoacid as crosslinkers, including l-glutamic acid, adipic acid, malonic acid, and levulinic acid. The products were characterized over their physicochemical and biological properties. FT–IR analysis confirmed formation of amide bonds. The scaffolds had a highly porous structure, which was confirmed by SEM analysis. Their porosity was above 90%. The biomaterials had excellent swelling abilities and very good antioxidant properties. The cytotoxicity study was performed on vaginal epithelial VK2/E6E7 and human colon cancer HCT116 cell lines. The results showed that after certain modifications, the proposed scaffolds could be used in pelvic organ prolapse (POP) treatment.
- Record ID
- CUTa86a92cf15c44b8e9f10eebd57d42eda
- Publication categories
- ;
- Author
- Journal series
- Molecules, ISSN , e-ISSN 1420-3049, Biweekly
- Issue year
- 2020
- Vol
- 25
- No
- 18
- Pages
- [1-15]
- Article number
- 4280
- Other elements of collation
- fot.; tab.; wykr.; Bibliografia (na s.) - 13-15; Bibliografia (liczba pozycji) - 31; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 25, Iss. 18, Spec. Iss.
- Substantive notes
- Special Issue: Advances in Chitin and Chitosan Science
- Keywords in English
- biomaterials, chitosan, pelvic organ prolapse
- DOI
- DOI:10.3390/molecules25184280 Opening in a new tab
- URL
- https://www.mdpi.com/1420-3049/25/18/4280 Opening in a new tab
- Related project
- Synteza i badanie właściwości nowych termoplastycznych pochodnych chitozanu o kontrolowanej biodegradowalności. . Project leader at PK: , ,
Projects financed by NSC [Projekty finansowane przez NCN] - Language
- eng (en) English
- License
- Score (nominal)
- 140
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTa86a92cf15c44b8e9f10eebd57d42eda/
- URN
urn:pkr-prod:CUTa86a92cf15c44b8e9f10eebd57d42eda
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.