Cell viability and collagen deposition on hydroxyapatite coatings formed on pretreated substrates
Authors:
- A. Yanovska,
- Ye Husak,
- O. Mishchenko,
- A. Gudakov,
- O. Oleshko,
- A. Yusupova,
- M. Vielikov,
- J. Radwan-Pragłowska,
- M. Piątkowski,
- Ł. Janus,
- E. Szajna,
- M. Pogorielov
Abstract
A method of thermal deposition that involves localized high peak temperatures at the electrode-electrolyte interface was proposed for hydroxyapatite (HA) coatings deposition. Active surface layers were obtained by HA incorporation from the aqueous electrolyte into the substrate surface due to the decrease of HA solubility with increasing of substrate temperature. Commercially Ti-6Al-4V alloy (grade 4) substrates were pretreated by sandblasting combined with various chemical pre-treatments: 20% NaOH, 20% oxalic acid, and both. After both types of pre-treatment and HA thermal deposition, we can observe uniform coatings with a rough surface. HA presence was confirmed by X-ray Diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) analysis. According to received data, samples after 6.5 bar, one cycle of sandblasting and etching in 20% oxalic acid 2 h, 20% H3PO4 1 min as well as untreated samples showed the highest wettability. All measured contact angles are lower than 90°, which means that obtained HA surfaces are hydrophilic and indicates that the wetting of the surface is favorable, and the fluid will spread over a large area of the surface. Resazurin reduction assay showed satisfactory U2OS osteoblastic cell adhesion in 1 day of the experiment. There is no significant difference between non-treated polished surfaces and HA-covered with sandblasting pre-treatment. In contrast – the polished surface with HA coating demonstrates significantly less cell adhesion. It was confirmed low collagen production both in untreated and polished-HA surfaces in comparison with the sandblasted-HA ones. The obtained data suggest that roughness plays an important role in cell adhesion and proliferation, but HA coating provides additional stimuli for cell activity (collagen synthesis).
- Record ID
- CUT623d4774ba4042b481595957367c768d
- Publication categories
- ;
- Author
- Journal series
- Materials Chemistry and Physics, ISSN 0254-0584, e-ISSN 1879-3312
- Issue year
- 2021
- Vol
- 258
- Pages
- [1-8]
- Article number
- 123978
- Other elements of collation
- fot.; tab.; wykr.; Bibliografia (na s.) - 8; Bibliografia (liczba pozycji) - 37; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 258
- Keywords in English
- chemical pre-treatment, collagen production, hydroxyapatite coatings, thermal deposition
- DOI
- DOI:10.1016/j.matchemphys.2020.123978 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/abs/pii/S0254058420313389 Opening in a new tab
- Language
- eng (en) English
- Score (nominal)
- 70
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT623d4774ba4042b481595957367c768d/
- URN
urn:pkr-prod:CUT623d4774ba4042b481595957367c768d
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.