Measurement methods for the mechanical testing and biocompatibility assessment of polymer-ceramic connective tissue replacements
Authors:
- K. Pluta,
- W. Florkiewicz,
- D. Malina,
- K. Rudnicka,
- S. Michlewska,
- J. B. Królczyk,
- A. Sobczak-Kupiec
Abstract
This paper describes the measurement methods of UV cross-linked hydroxyapatite-polymers composites particularly focused on the role of their composition on structure, mechanical properties, and biocompatibility. Morphology and elemental composition were investigated with SEM-EDS. The chemical structure was analysed with FT-IR spectroscopy. Stereometric and surface roughness parameters, stress and strain in the static tensile test, and swelling kinetic of composites were also studied. Biocompatibility assessment was performed utilizing direct contact cytotoxicity assay and pro-inflammatory tests. Cell morphology observation was undertaken with confocal microscopy. The composites containing poly(vinylpyrrolidone) and gelatin revealed a 9% deformation and 164.0 ±2.3% sorption capacity. The addition of albumin and aspartic acid results in a 16% higher defor-mation and 166.5 ±3.3% equilibrium swelling. The materials were not cytotoxic. The morphology of the cells exposed to the composites remained unchanged. Composite based on poly(vinylpyrrolidone) and gelatin induced monocyte cytoplasmic extensions lengthening of 207.2% in comparison with the reference material.
- Record ID
- CUT28fb63876383470092ac8ccc73c889f7
- Publication categories
- ;
- Author
- Journal series
- Measurement, ISSN 0263-2241, e-ISSN 1873-412X
- Issue year
- 2021
- Vol
- 171
- Pages
- [1-14]
- Article number
- 108733
- Other elements of collation
- fot.; schem.; tab.; wykr.; Bibliografia (na s.) - 13-14; Bibliografia (liczba pozycji) - 53; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 171
- Keywords in English
- mechanical testing, biocompatibility assessment, biocomposites, hydroxyapatite
- DOI
- DOI:10.1016/j.measurement.2020.108733 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/abs/pii/S0263224120312379 Opening in a new tab
- Related project
- Innowacyjne biomateriały kompozytowe zawierające bioaktywne fosforany wapnia. . Project leader at PK: , ,
Projects financed by NCRD [Projekty finansowane przez NCBiR (NCBR (] - Language
- eng (en) English
- Score (nominal)
- 200
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT28fb63876383470092ac8ccc73c889f7/
- URN
urn:pkr-prod:CUT28fb63876383470092ac8ccc73c889f7
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.