Fluorescence assay for the determination of D-panthenol based on novel ring-fused 2-pyridone derivative
Authors:
- Wiktor Kasprzyk,
- Tomasz Świergosz,
- Filip Koper
Abstract
Herein, a novel fluorescent method for the determination of d-panthenol (DP) level in solutions with no separate hydrolysis step has been revealed based on the utilization of citric acid (CA) as a derivatizing agent. Consequently, the essential parameters of the derivatization process were established, resulting in the development of sensitive, repeatable, and accurate determination of panthenol. The method was approved, and its usefulness in characterizing the concentration of DP in pharmaceutical formulations and selectivity in the determination of DP were validated. The chemical structure of the new fluorophore formulating in the reaction in DP with CA, i.e., 6-oxo-3,4-dihydro-2H,6H-pyrido[2,1-b][1,3]oxazine-8-carboxylic acid (ODPC), was elucidated using detailed NMR experiments: one-dimensional (1H, 13C) as well as two-dimensional NMR spectra ( 1H-1H COSY, 1H-13C HSQC, 1H-13C HMBC, 1H-15N HSQC, 1H-15N HMBC).
- Record ID
- CUT8b28840465e441d881ee1a13dfa18e07
- Publication categories
- ;
- Author
- Journal series
- International Journal of Molecular Sciences, ISSN 1661-6596, e-ISSN 1422-0067, Biweekly
- Issue year
- 2020
- Vol
- 21
- No
- 21
- Pages
- [1-12]
- Article number
- 8386
- Other elements of collation
- schem.; tab.; wykr.; Bibliografia (na s.) - 10-12; Bibliografia (liczba pozycji) - 44; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 21, Iss. 21
- Substantive notes
- Art. zawiera: Supplementary Materials
- Section: Molecular Pharmacology
- Keywords in English
- d-panthenol, citric acid, fluorescence, derivatization, ring-fused 2-pyridones
- DOI
- DOI:10.3390/ijms21218386 Opening in a new tab
- URL
- https://www.mdpi.com/1422-0067/21/21/8386/htm Opening in a new tab
- Related project
- Nowe fluorescencyjne poliestry o właściwościach przeciwutleniających do zastosowań w inżynierii naczyń krwionośnych. . Project leader at PK: , ,
Projects financed by NSC [Projekty finansowane przez NCN] - Language
- eng (en) English
- License
- Score (nominal)
- 140
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT8b28840465e441d881ee1a13dfa18e07/
- URN
urn:pkr-prod:CUT8b28840465e441d881ee1a13dfa18e07
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.