Semi-targeted metabolomics to validate biomarkers of grape downy mildew infection under field conditions
Authors:
- Kévin Billet,
- Magdalena Anna Malinowska,
- Thibaut Munsch,
- Marianne Unlubayir,
- Sophie Adler,
- Guillaume Delanoue,
- Arnaud Lanoue
Abstract
Grape downy mildew is a devastating disease worldwide and new molecular phenotyping tools are required to detect metabolic changes associated to plant disease symptoms. In this purpose, we used UPLC-DAD-MS-based semi-targeted metabolomics to screen downy mildew symptomatic leaves that expressed oil spots (6 dpi, days post-infection) and necrotic lesions (15 dpi) under natural infections in the field. Leaf extract analyses enabled the identification of 47 metabolites belonging to the primary metabolism including 6 amino acids and 1 organic acid, as well as an important diversity of specialized metabolites including 9 flavonols, 11 flavan-3-ols, 3 phenolic acids, and stilbenoids with various degree of polymerization (DP) including 4 stilbenoids DP1, 8 stilbenoids DP2, and 4 stilbenoids DP3. Principal component analysis (PCA) was applied as unsupervised multivariate statistical analysis method to reveal metabolic variables that were a ected by the infection status. Univariate and multivariate statistics revealed 33 and 27 metabolites as relevant infection biomarkers at 6 and 15 dpi, respectively. Correlation-based networks highlighted a general decrease of flavonoid-related metabolites, whereas stilbenoid DP1 and DP2 concentrations increased upon downy mildew infection. Stilbenoids DP3 were identified only in necrotic lesions representing late biomarkers of downy mildew infection.
- Record ID
- CUT52a8b071b011408581f01429009eefef
- Publication categories
- ;
- Author
- Journal series
- Plants, ISSN , e-ISSN 2223-7747, Biweekly
- Issue year
- 2020
- Vol
- 9
- No
- 8
- Pages
- [1-18]
- Article number
- 1008
- Other elements of collation
- fot.; schem.; tab.; wykr.; Bibliografia (na s.) - 15-18; Bibliografia (liczba pozycji) - 51; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 9, Iss. 8, Spec. Iss.
- Substantive notes
- Special Issue: Resveratrol in Plants
- Keywords in English
- grape, downy mildew, semi-targeted metabolomics, infection biomarkers, polyphenols, stilbenoids, correlation network
- DOI
- DOI:10.3390/plants9081008 Opening in a new tab
- URL
- https://www.mdpi.com/2223-7747/9/8/1008/htm Opening in a new tab
- Language
- eng (en) English
- License
- Score (nominal)
- 70
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT52a8b071b011408581f01429009eefef/
- URN
urn:pkr-prod:CUT52a8b071b011408581f01429009eefef
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.