Chitosan-based bioactive hemostatic agents with antibacterial properties—synthesis and characterization
Authors:
- Julia Radwan-Pragłowska,
- Marek Piątkowski,
- Volodymyr Deineka,
- Łukasz Janus,
- Viktoriia Korniienko,
- Evgenia Husak,
- Viktoria Holubnycha,
- Iryna Liubchak,
- Vyacheslav Zhurba,
- Aleksandra Sierakowska,
- Maksym Pogorielov,
- Dariusz Bogdał
Abstract
Massive blood loss is responsible for numerous causes of death. Hemorrhage may occur on the battlefield, at home or during surgery. Commercially available biomaterials may be insufficient to deal with excessive bleeding. Therefore novel, highly efficient hemostatic agents must be developed. The aim of the following research was to obtain a new type of biocompatible chitosan-based hemostatic agents with increased hemostatic properties. The biomaterials were obtained in a quick and efficient manner under microwave radiation using l-aspartic and l-glutamic acid as crosslinking agents with no use of acetic acid. Ready products were investigated over their chemical structure by FT-IR method which confirmed a crosslinking process through the formation of amide bonds. Their high porosity above 90% and low density (below 0.08 g/cm3) were confirmed. The aerogels were also studied over their water vapor permeability and antioxidant activity. Prepared biomaterials were biodegradable in the presence of human lysozyme. All of the samples had excellent hemostatic properties in contact with human blood due to the platelet activation confirmed by blood clotting tests. The SEM microphotographs showed the adherence of blood cells to the biomaterials’ surface. Moreover, they were biocompatible with human dermal fibroblasts (HDFs). The biomaterials also had superior antibacterial properties against both Staphylococcus aureus and Escherichia coli. The obtained results showed that proposed chitosan-based hemostatic agents have great potential as a hemostatic product and may be applied under sterile, as well as contaminated conditions, by both medicals and individuals.
- Record ID
- CUT55a16ba68de54a499926133d05374498
- Publication categories
- ;
- Author
- Journal series
- Molecules, ISSN , e-ISSN 1420-3049, Biweekly
- Issue year
- 2019
- Vol
- 24
- No
- 14
- Pages
- [1-17]
- Article number
- 2629
- Other elements of collation
- fot.; rys.; tab.; wykr.; Bibliografia (na s.) - 16-17; Bibliografia (liczba pozycji) - 36; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 24, Iss. 14, Spec. Iss.
- Substantive notes
- Spec. Iss.: Advances in Chitin and Chitosan Science
- Keywords in English
- antibacterial biomaterials, chitosan, green chemistry, hemostatic agents, polymeric materials
- DOI
- DOI:10.3390/molecules24142629 Opening in a new tab
- URL
- https://www.mdpi.com/1420-3049/24/14/2629 Opening in a new tab
- Related project
- Language
- eng (en) English
- License
- Score (nominal)
- 140
- Publication indicators
- = 56
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT55a16ba68de54a499926133d05374498/
- URN
urn:pkr-prod:CUT55a16ba68de54a499926133d05374498
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.