Copper(II) complexes of coumarin-derived Schiff base ligands: Pro- or antioxidant activity in MCF-7 cells?
Authors:
- Louise MacLean,
- Dariusz Karcz,
- Hollie Jenkins,
- Siobhán McClean,
- Michael Devereux,
- Orla Howe,
- Marcos D. Pereira,
- Nóra V. May,
- Éva A. Enyedy,
- Bernadette S. Creaven
Abstract
A series of copper(II) complexes of Schiff base-derived ligands (1–7) were studied for their pro- and antioxidant behaviour in the MCF-7 human breast cancer cell line. The coordination modes of two of the copper(II) complexes were investigated by pH-potentiometry, EPR and UV–Vis spectroscopic methods. The solution studies indicated that monomeric species are present in the Cu(II) – L1 system at neutral pH, whereas dinuclear species were observed in the case of the Cu(II) – L7 system. This difference in speciation was reflected in their relative cytotoxicities with the copper(II) complex of L1, showing significant cytotoxicity against MCF-7 cells whilst the complex of L7 was inactive. In fact, only three of the seven complexes studied in this series were cytotoxic to MCF-7 cells but this cytotoxicity did not correlate with their ability to bind to DNA, cleave DNA or act as a pro-oxidant. In contrast to previous copper(II) complexes studied by our group, the compounds studied here do not appear to lead to intracellular reactive oxygen species generation at any significant level. In a yeast-based assay, all of the copper complexes had the ability to protect Saccharomyces cerevisiae against menadione-induced oxidative stress but not hydrogen peroxide-induced stress, indicating a lack of catalase activity. Given that the adaptive mechanisms induced by hypoxia in cancer cells have selective effects, with a fine-tuned protection against damage and stress of many kinds, particularly against oxidative stress, chemotherapeutic compounds which are not pro-oxidants may offer a therapeutic advantage.
- Record ID
- CUT8d962948155045ab8ff5100c527be93c
- Publication categories
- ;
- Author
- Journal series
- Journal of Inorganic Biochemistry, ISSN 0162-0134, e-ISSN 1873-3344
- Issue year
- 2019
- Vol
- 197
- Pages
- [1-14]
- Article number
- 110702
- Other elements of collation
- rys.; tab.; wykr.; Bibliografia (na s.) - 14; Bibliografia (liczba pozycji) - 46; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 197
- Keywords in English
- copper complexes, Schiff bases, ROS, speciation
- DOI
- DOI:10.1016/j.jinorgbio.2019.110702 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/pii/S0162013418306639 Opening in a new tab
- Language
- eng (en) English
- Score (nominal)
- 100
- Additional fields
- Indeksowana w: Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT8d962948155045ab8ff5100c527be93c/
- URN
urn:pkr-prod:CUT8d962948155045ab8ff5100c527be93c
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.