Puzzling aqueous solubility of guanine obscured by the formation of nanoparticles
Authors:
- Termeh Darvishzad,
- Tomasz Lubera,
- Stefan S. Kurek
Abstract
Dissolution of guanine in neutral solutions was obscured by peculiar behavior of guanine, indicating an apparent dependence of solubility on the amount of solid guanine used. Here, we demonstrate that the problem is caused by the formation of tiny guanine nanoparticles that tend to grow forming stable particles of ca. 800 nm size. This effect can be minimalized by using small quantities of guanine powder for dissolution. We also show that assuming a constant, independent of pH, concentration of neutral form of guanine, at 25 °C equal 25.4 μM, and applying known pKa values related to its dissociation or protonation, it is possible to calculate the concentrations of all conjugate acids and bases of guanine at the given pH value, and by summing them up, the guanine solubility.
- Record ID
- CUT8335a25a76c249c980552cc33d2dac25
- Publication categories
- ;
- Author
- Journal series
- Journal of Physical Chemistry B, ISSN 1520-6106, e-ISSN 1520-5207
- Issue year
- 2018
- Vol
- 122
- No
- 30
- Pages
- 7497-7502
- Other elements of collation
- tab.; wykr.; Bibliografia (na s.) - 7502; Bibliografia (liczba pozycji) - 23; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 122, Iss. 30
- Keywords in English
- guanine, aqueous solubility, nanoparticles
- DOI
- DOI:10.1021/acs.jpcb.8b04327 Opening in a new tab
- URL
- https://pubs.acs.org/doi/10.1021/acs.jpcb.8b04327 Opening in a new tab
- Language
- eng (en) English
- Score (nominal)
- 30
- Publication indicators
- = 16
- Citation count
- 16
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT8335a25a76c249c980552cc33d2dac25/
- URN
urn:pkr-prod:CUT8335a25a76c249c980552cc33d2dac25
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.