Separation of betacyanins from purple flowers of Gomphrena globosa L. by ion-pair high-speed counter-current chromatography
Authors:
- Aneta Spórna-Kucab,
- Joanna Jagodzińska,
- Sławomir Wybraniec
Abstract
Betacyanins, known as antioxidants and chemopreventive natural compounds with colourful properties, were extracted from purple flowers of Gomphrena globosa L. belonging to the Amaranthaceae family and separated for the first time by ion-pair high-speed counter-current chromatography (HSCCC). The pigments were detected by LC-DAD-ESI-MS/MS technique. Separation of betacyanins (300 mg) by HSCCC was accomplished in four solvent systems: tert-butyl methyl ether – butanol – acetonitrile – water (0.7% and 1.0% HFBA – heptafluorobutyric acid – system I and III) and tert-butyl methyl ether – butanol – methanol – water (0.7% and 1.0% HFBA – system II and IV) (2:2:1:5, v/v/v/v) in the head-to-tail mode. The mobile phase (aqueous phase) was run at 2.0 ml/min and the column rotation speed was 860 rpm. The applied systems enabled to study the influence of HFBA concentration as well as systems polarity on betacyanins separation. Comparison of the systems containing 0.7% HFBA (systems I–II) demonstrates that the replacement of acetonitrile by methanol increases the resolution (Rs) between all betacyanins and does not influence the retention of the stationary phase (Sf = 76%). Higher concentration of the acid in systems III–IV slightly decreases Sf to 71% in the systems with 1.0% HFBA. Comparison of the resolution values for betacyanins in the systems with 0.7% and 1.0% HFBA demonstrates that higher concentration of the acid improves the separation effectiveness for all betacyanins as a result of increasing of the chemical affinity of the pigments to the organic stationary phase in HSCCC. The systems III–IV with 1% HFBA are the most effective for the separation of all the studied betacyanins.
- Record ID
- CUTe0c8ad8a66ef4732af2d7f89fe45fa37
- Publication categories
- ;
- Author
- Journal series
- Journal of Chromatography A, ISSN 0021-9673, e-ISSN 1873-3778
- Issue year
- 2017
- Vol
- 1489
- Pages
- 51-57
- Other elements of collation
- tab.; wykr.; Bibliografia (na s.) - 57; Bibliografia (liczba pozycji) - 34; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 1489
- Keywords in English
- betacyanins, ion-pair reagents, counter-current chromatography, Gomphrena globosa L.
- DOI
- DOI:10.1016/j.chroma.2017.01.064 Opening in a new tab
- URL
- http://www.sciencedirect.com/science/article/pii/S0021967317301498 Opening in a new tab
- Related project
- Badania mechanizmu utleniania pigmentów betacyjanin. . Project leader at PK: , ,
Projects financed by NSC [Projekty finansowane przez NCN] - Language
- eng (en) English
- Score (nominal)
- 40
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTe0c8ad8a66ef4732af2d7f89fe45fa37/
- URN
urn:pkr-prod:CUTe0c8ad8a66ef4732af2d7f89fe45fa37
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