Studies on polar high-speed counter-current chromatographic systems in separation of amaranthine-type betacyanins from Celosia species
Authors:
- Aneta Spórna-Kucab,
- Angelika Milo,
- Agnieszka Kumorkiewicz,
- Sławomir Wybraniec
Abstract
Betacyanins, natural plant pigments exhibiting antioxidant and chemopreventive properties, were extracted from Celosia spicata (Thouars) Spreng. inflorescences and separated by high-speed counter-current chromatography (HSCCC) in two polar solvent systems composed of: TBME – 1-BuOH – ACN – H2O (0.7% HFBA, 2:2:1:5, v/v/v/v) (system I) and EtOH – ACN – 1-PrOH – (NH4)2SO4satd.soln – H2O (0.5:0.5:0.5:1.2:1, v/v/v/v/v) (system II). The systems were used in the head-to-tail (system I) and tail-to-head (system II) mode. The flow rate of the mobile phase was 2.0 ml/min and the column rotation speed was 860 rpm. The retention of the stationary phase was 73.5% (system I) and 80.0% (system II). For the identification of separated betacyanins in the crude extract as well as in the HSCCC fractions, LC-DAD-ESI–MS/MS analyses were performed. Depending on the target compounds, each of the systems exhibit meaningfully different selectivity and applicability. For the pairs of amaranthines (1/1′) and betanins (2/2′), the best choice is the system II, but the acylated amaranthine pairs (3/3′ and 4/4′) can be resolved only in the ion-pair system I. For the indication of the most suitable solvent system for Celosia plumosa hort., Celosia cristata L. and Celosia spicata (Thouars) Spreng. species, the profiles of betacyanins in different plant parts were studied.
- Record ID
- CUTf72ca3e6e28c494c83ffdb6fd6047d3b
- Publication categories
- ;
- Author
- Journal series
- Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, ISSN 1570-0232, e-ISSN 1873-376X
- Issue year
- 2018
- Vol
- 1073
- Pages
- 96-103
- Other elements of collation
- tab.; wykr.; Bibliografia (na s.) - 103; Bibliografia (liczba pozycji) - 35; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 1073
- Keywords in English
- Celosia, betacyanins, amaranthine, betanin, HSCCC, Gomphrena globosa L.
- DOI
- DOI:10.1016/j.jchromb.2017.11.028 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/pii/S157002321731365X Opening in a new tab
- Related project
- Badania mechanizmu utleniania pigmentów betacyjanin. . Project leader at PK: , ,
Projects financed by NSC [Projekty finansowane przez NCN] - Language
- eng (en) English
- Score (nominal)
- 30
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUTf72ca3e6e28c494c83ffdb6fd6047d3b/
- URN
urn:pkr-prod:CUTf72ca3e6e28c494c83ffdb6fd6047d3b
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