Anti- and pro-oxidative activity measured directly as the extent of 8-oxoguanine production
Authors:
- Termeh Darvishzad,
- Stefan S. Kurek
Abstract
An electrochemical method was developed for the evaluation of antioxidant activity based on the ability to inhibit 8-oxoguanine production. It consists of a sequence of successive potential hold and scan steps, in which superoxide anion radical is generated by the reduction of dioxygen in a potential hold step in a buffer solution containing guanine and antioxidant, immediately followed by a potential scan step, in which 8-oxoguanine is detected and its amount determined. The effect of 11 antioxidants, among them simple alcohols for comparison, was investigated in pH 7 and pH 9 phosphate buffers saturated with guanine. The plots of 8-oxoguanine peak height, expressed as percent of the peak current in the absence of antioxidants, versus antioxidant concentration showed an exponential decay character. The decay parameter may be used as a measure of antioxidant activity. It is directly related to the IC50 coefficient used in other methods Only simple alcohols displayed single decay character. Other studied substances showed multi-exponential decay at pH 7, most investigated antioxidants were unable to entirely inhibit the generation of 8-oxoguanine, but led to a steady state of 8-oxoguanine level. At pH 9, many antioxidants, particularly polyphenolics and ascorbyl phosphate, showed pure pro-oxidant activity.
- Record ID
- CUT615d9ac98ce3466ebb839662161c3774
- Publication categories
- ;
- Author
- Journal series
- Electrochimica Acta, ISSN 0013-4686, e-ISSN 1873-3859
- Issue year
- 2017
- Vol
- 240
- Pages
- 466-473
- Other elements of collation
- wykr.; Bibliografia (na s.) - 473; Bibliografia (liczba pozycji) - 37; Oznaczenie streszczenia - Abstr.; Numeracja w czasopiśmie - Vol. 240
- Keywords in English
- antioxidant activity, 8-oxoguanine, polyphenolics, ROS, voltammetry
- DOI
- DOI:10.1016/j.electacta.2017.04.074 Opening in a new tab
- URL
- http://www.sciencedirect.com/science/article/pii/S0013468617308332 Opening in a new tab
- Language
- eng (en) English
- Score (nominal)
- 40
- Additional fields
- Indeksowana w: Web of Science, Scopus
- Uniform Resource Identifier
- https://cris.pk.edu.pl/info/article/CUT615d9ac98ce3466ebb839662161c3774/
- URN
urn:pkr-prod:CUT615d9ac98ce3466ebb839662161c3774
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.